Publications > Vasilev et al
Synthesis of novel tetracationic asymmetric monomeric monomethine cyanine dyes – highly fluorescent dsDNA probes
Resumen
Quaternisation of monomethine cyanine dyes bearing haloalkyl chains, with the appropriate monoquaternised 1,4-diazabicyclo%@5B2.2.2%@5Doctane derivatives, yielded novel tetracationic asymmetric monomeric monomethine cyanine dyes belonging to the thiazole orange family. The optimal conditions for this reaction were investigated. The longest wavelength absorption maxima of the studied dyes are in the region 504?507?nm. The molar absorptivities of the target dyes are high, with values between 70?500 and 99?500?l?mol?1?cm?1. The fluorescence intensity and the fluorescence quantum yields of the free dyes in solution or in the presence of double-stranded DNA were determined using thiazole orange for comparison. The dyes are suitable for nucleic acid detection and have a high binding affinity, attributable to their four positive charges.